While your OT-2 can be adapted to use a wide range of labware, we've built this as a guide to labware that's been tested for compatibility and is ready to work with right out of the box. Simple liquid handling. skills, You received your robot, here's what's next, Examples in this section expect the following, opentrons_10_tuberack_falcon_4x50ml_6x15ml_conical. Deck slot numbers play no role in the ordering of modules; you could load a Temperature Module in slot 4 first, followed by another one in slot 3: For this code to work as expected, temperature_module_1 should be plugged into a lower-numbered USB port than temperature_module_2. below, both lines refer to well 'A1'. Our workflow makes use of custom-designed 3D-printed labware, the relatively inexpensive OpenTrons liquid handling platform, and recently developed methods for Illumina library preparation using highly diluted reagents. Please test before carrying out your experiment to ensure the desired engage height for your labware. The Opentrons API comes with many common labware built in. We recommend you switch over to using the load names from the Labware Library long. When writing a protocol using the API, you will need to select which wells to when we find an issue with a labware definition. especially with irregular labware (e.g. Its also why many researchers have created customized 3D printed labware and accessories for the OT-2. Our open-source liquid handler supports various biochemical assays for top applications in genomics, proteomics, and drug discovery including PCR, qPCR, RT-PCR, next-generation sequencing (NGS), synthetic biology, protein purification, sample & library preparation . A labwares wells are organized within a series of columns and rows, which are The Heater-Shaker Module provides on-deck heating and orbital shaking. to work with the older definition. unsupported format. Opentrons standard labware library. Having an Opentrons business account allows you to: Pay on terms; Add purchasers and admins to your company profile; Pay invoices online on the Opentrons portal . The deep-well bracket can support deep well trays up to 47 mm tall. Labware.wells_by_name(): Wells can be referenced by their name, as demonstrated above. Get regular updates on our latest products and newest educational articles. Well objects have methods that provide information about their physical shape, such as the depth or diameter, as specified in their corresponding labware definition. These will return all wells within that row A protocol, in its most basic form: Provides some information about who made the protocol and what it is for. At some points in your protocol, you may want to deactivate specific temperature controllers of your Thermocycler. Running protocols with multiple modules of the same type requires version 4.3 or newer of the Opentrons App and OT-2 robot server. Check out the documentation and start writing your own protocols. Opentrons publishes new versions of a labware definition In the example above, the call to labware.create The OT-2 deck and labware are all set up with the same coordinate system. especially with irregular labware (e.g. Any custom labware added to your Opentrons App (see Custom Labware) is accessible when loading labware onto a module. NOTE: We embrace these tools but cannot endorse or validate their efficacy or compatibility with the Opentrons Labware Library. There are many different ways to access wells inside labware. skills, You received your robot, here's what's next, Examples in this section require the following, 'opentrons_96_aluminumblock_biorad_wellplate_200ul', # version 2 of the aluminum block definition, # version 1 of the aluminum block definition, # if you don't specify version, version 1 is used, # distances (mm) between each (column, row), Examples in this section expect the following, # slice until index 8, without including it. Or, we can get a group of wells and loop Attempting to load tall labware to the right or left of the Heater-Shaker will also raise a DeckConflictError. In the API, rows are given letter names If you need assistance creating custom labware definitions for the Heater-Shaker, submit a request. "B3") to be easier to reason with, well shape. {'A': [labware:A1, labware:A2..], 'B': [labware:B1, labware:B2]}, Dictionary with column names as keys, i.e. It can be one of the strings 'holding at target', 'cooling', 'heating', 'idle', or 'error'. a labware being at position 0. The current status of the lid position. Contact our Application Engineers). subsequent protocol runs. Define and track your reagents and samples. Width is defined as the distance along the y-axis (front to back). Its up to you to make sure that the labware and module you chose make sense together. The rows() or cols() methods can be used in combination with the Get the latest updates on new products and upcoming sales. Compare this with similar-looking commands for holding a sample at a temperature for one minute: This may take much longer, depending on the thermal block used, the volume and type of liquid contained in the labware, and the initial temperature of the module. Use Well.diameter() to get the diameter of a given well in mm. V-bottom. (for example: to make changes to its definition), you can do the following: There is some specialty labware that will require you to specify the In order to send commands to the correct module on the deck, you need to load the modules in your protocol in a specific order. {'A1': labware:A1, 'B1': labware:B1}, Dictionary with row names as keys, i.e. This prevents the Heater-Shakers latch from colliding with the adjacent labware. Meet the scientists The standard plate bracket supports plates up to 22 mm tall. The current status of the well block temperature controller. You can control the lid position with the methods below. The recommended way to specify the magnets position is to utilize the height_from_base parameter, which allows you to raise the height of the magnets relative to the base of the labware. If a well does not exist in this labware, It also takes a block_max_volume parameter, which is the same as that of the ThermocyclerContext.set_block_temperature() function. You are currently viewing the documentation for version 1 of the Magnetic Module, labware, Swift Bioscience library prep kit, and Omega Bio-tek magnetic beads. Hardware modules are first-party peripherals that attach to the OT-2 to extend its capabilities. To view version 2 of the API, [labware:A1, labware:B1, labware:C1], List of a list ordered by row, i.e [[labware:A1, labware:A2], [labware:B1, labware:B2..]], List of a list ordered by column, i.e. If you do not specify a version when loading labware, version 1 will be used by default. Next, you cant place tall labware (defined as >53 mm) to the left or right of the Heater-Shaker. If a labware that does not have a defined engage height is loaded on the Magnetic Module (or if no labware is loaded), then height_from_labware (since version 2.2) or height, must be specified. The OT-2 will not automatically deactivate the Heater-Shaker at the end of a protocol. document.write(new Date().getFullYear()), Gain an overview of our protocol creation options, Use our graphical user interface to design protocols, Explore our open source database of protocols, Maximum customization for anyone with python and basic The design goal of this API is to make code readable and easy to understand. Sometimes, you may need to place a labware on top of something else on the It can be one of the strings 'open', 'closed' or 'in_between'. List of all wells, i.e. In API version 2.1 (introduced in Robot Software version 3.15.2) or higher you can now specify a label, version, and namespace (though most of the time you wont have to). When writing a protocol, you must inform the Protocol API about the labware you will be placing on the OT-2s deck. The Thermocycler can rapidly cycle through temperatures to execute heat-sensitive reactions. with the same load name. If the well is not rectangular, the value returned will be None. Copy and paste load names directly from the Labware Library to ensure If you are creating a custom tip rack, it Columns of wells (see image below) on a labware are typically labeled with numerical indices starting with '1'; The ending well will be in the bottom right, see the diagram below for further explanation. Learn more about OT-2 Python Protocol API Version 2 here . The labware on your robot was selected for compatibility with the OT-2 deck and pipettes, and has been tested both in-house and in our customers' labs. Currently, only the following combinations are supported in the Opentrons Labware Library: opentrons_96_deep_well_adapter_nest_wellplate_2ml_deep, opentrons_96_flat_bottom_adapter_nest_wellplate_200ul_flat, NEST 96 Well Plate 100 L PCR Full Skirt, opentrons_96_pcr_adapter_nest_wellplate_100ul_pcr_full_skirt, opentrons_96_pcr_adapter_armadillo_wellplate_200ul, opentrons_universal_flat_adapter_corning_384_wellplate_112ul_flat. Phone: (425) 888-1223. Once a labware is loaded into your protocol, you can easily access the many Sign up for our newsletter. All Blocks Well Plates Well Reservoirs 4-in-1 Tube Rack Set $220.00. Again, if you would like to add any of these tools added to our Labware Library, or you need to add other custom labware, please fill out this form. When you load labware, you specify the name of the labware (e.g. definitions. Note: This labware definition requires Opentrons software v5.0 or later. See our Applications Expertise and Benchmark Solutions. deactivate if another protocol is uploaded to the app. When loading the Thermocycler, it is not necessary to specify a slot. labware in a certain slot. Use ProtocolContext.load_module() to load a module. document.write(new Date().getFullYear()); OPENTRONS There is one exception: to the front or back of the Heater-Shaker, an 8-channel pipette can access tip racks only. ThermocyclerContext.set_lid_temperature() takes one parameter: the temperature you wish the lid to be set to. of its deck between 4 C and 95 C with a resolution of 1 C. How do I know if I have Opentrons tips? Our open-source liquid handler supports various biochemical assays for top applications in genomics, proteomics, and drug discovery including PCR, qPCR, RT-PCR, next-generation sequencing (NGS), synthetic biology, protein purification, sample & library preparation . You may find well names (e.g. created through this method must consist of circular wells arranged in You should set a lid temperature before executing the profile using ThermocyclerContext.set_lid_temperature(). Count- The total number of tubes or wells in your labware. Examples of such compatible labware include: Bio-Rad 96 Well Plate 200 L PCR For instance, you can execute the profile defined above 100 times for a 30 L-per-well volume like this: Temperature profiles only control the temperature of the block in the Thermocycler. Bio-Rad 96 Well Plate 200 L PCR also labelled on standard labware. These methods both return a dictionary with the row or column name as the keys: Since these methods return either lists or dictionaries, you can iterate through them as you would regular Python data structures. Rows, which are also prepared to take string values for the protocol API wont you Left or right of the labware parameters, ramp_rate and block_max_volume are.. And/Or removal, please visit the Opentrons API, click here basic form: opentrons labware library some about! The target is reached a href= '' https: //opentrons.com/protocols/designer/ '' > Designer. Disengage when the opentrons labware library temperature before executing a Thermocycler profile ( see custom labware process here accurate volume the. If your protocol Collaborate and Share - Opentrons < /a > 7824 center Boulevard SE comes many To understand all labware load names that should be passed in as or Referenced with zero-indexing, with the methods available to protocols and so on open or closed modifications be. You must inform the OT-2 deck Opentrons automation system ensure the desired engage height for your labware move.! 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