@vH3 ;:4 0000049487 00000 n Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Combine an equal amount of amplicon from each sample (240 ng) into a single, sterile tube. (2011) Removing noise from pyrosequenced amplicons. 0000041328 00000 n Parada, A. E., Needham, D. M., & Fuhrman, J. endstream endobj 40 0 obj <> endobj 41 0 obj <> endobj 42 0 obj <>stream no. Customer Dashboard, Infrastructure The updated, forward-barcoded primer sequences (Parada, Apprill) were implemented by the Knight Lab starting in 2015. . Illumina sequencing and array technologies fuel advancements in life science research, translational and consumer genomics, and molecular diagnostics. Vt A+MegcTa 0000220991 00000 n These bases were added to the 3 end of the Illumina 5 adapter sequence to increase the Tm for read 1 during sequencing. 0000054797 00000 n However, the B could be misinterpreted as barcode, and these names do not appear in the literature. 0000194132 00000 n Cycle times are longer for 384-well thermocyclers; these conditions should also work for 96-well thermocyclers. Enrichment reagents not included. The 16S Illumina Demonstrated Library Prep Guide and links to an example 16S dataset from libraries generated with the protocol and run on the MiSeq with v3 reagents. October 2018 Changed primer names to 515F (Parada), 806R (Apprill), and 926R (Quince). *Specifications for Illumina DNA Prep kit. Note: When working with multiple plates of samples, it is typical to produce a single tube of amplicons for each plate of samples. 0 0000162553 00000 n AC4vB|#By`mBP;XR/|3 ZERO BIAS - scores, article reviews, protocol conditions and more "C] ADYcPE The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). 0000082239 00000 n Software Suite, BaseSpace Pool triplicate PCR reactions for each sample into a single volume (75 L). 0000087769 00000 n Primers515F806R target the V4 region of the 16S SSU rRNA. Find out the benefits of each approach and how they differ. The. Find reagent kits, automation protocols, software solutions, and services to ensure your NovaSeq System is running the most efficiently. The Illumina 16S Metagenomic Sequencing Library Preparation Guide is an easy-to-follow protocol for preparing DNA libraries. 0000052110 00000 n 0000044007 00000 n 526 0 obj <> endobj xref Retailer Reg: 2019--2018 | Not for use in diagnostic procedures (except as specifically noted). 0000054719 00000 n 0000005067 00000 n 0000121016 00000 n 0000033475 00000 n 0000015942 00000 n It allows for multiplexing of up to 96 libraries per sequencing run using Illumina's dual indexing system. 0000014131 00000 n Tax Reg: 105-87-87282 | I2j}h Zl n`%nth e(* 0000082317 00000 n The amplicons are then purified for library preparation, after which, library quality control commences. 0000100694 00000 n The current primers have been modified from the original 515F806R primer pair (Caporaso et al., 2011) in the following ways: The primer sequences without linker, pad, barcode, or adapter are as follows: Note on primer names: Previously we had named the updated primers 515FB and 806RB to distinguish them from the original primers. 0000005339 00000 n 0000124676 00000 n 0000162514 00000 n 16S rRNA Sequencing is another method used for metagenomics studies. Degeneracy was added to both the forward and reverse primers to remove known biases against Crenarachaeota/Thaumarchaeota (515F, also called 515F-Y, Parada et al., 2016) and the marine and freshwater Alphaproteobacterial clade SAR11 (806R, Apprill et al., 2015). Illumina TruSeq PCR-Free Library Preparation Kit; . Illumina Demonstrated 16S Protocol describes a method for preparing samples for sequencing the variable V3 and V4 regions of the 16S rRNA gene. 0000057392 00000 n Our library preparation protocol was successfully validated on three sets of heterogeneous amplicons (16S rRNA gene amplicons from SPRI and PowerSoil extractions as well as control arthropod COI gene amplicons) that were sequenced across three independent, 250-bp, paired-end runs on Illumina's MiSeq platform. 0 The amplicon was purified using a XSEP MagBead (Celemics Inc., Seoul, Korea) and 300 bp paired-end sequencing using the MiSeq v3 platform (Illumina) was performed. Get instructions for sharing your desktop while working with Technical Support. Note for Qiita users: Studies in the Qiita database will havelibrary_construction_protocol as515f/806rbc if original primers or 515fbc/806r if current primers (bc stands for barcode). Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. The number of sequences per sample ranged from 70664 to 174456 (mean 121765, SD 35853). Unique molecular identifiers (UMIs) provide error correction and accuracy. The 11 soil DNA samples used for the pyrosequencing of 16S rRNA genes were also used for the metagenomic sequencing by the Illumina platform with a maximum read length of 75 bases (at the time of sequencing). AATGATACGGCGACCACCGAGATCTACACGCT XXXXXXXXXXXX TATGGTAATT GT GTGYCAGCMGCCGCGGTAA, CAAGCAGAAGACGGCATACGAGAT AGTCAGCCAG CC GGACTACNVGGGTWTCTAAT. 16S rRNA NGS allows microbiologists to achieve genus-level sensitivity for metagenomic surveys of bacterial populations. Metagenomics studies are useful for identifying the microbial species present in a sample. Caporaso, J. G., Lauber, C. L., Walters, W. A., Berg-Lyons, D., Huntley, J., Fierer, N., Owens, S. M., Betley, J., Fraser, L., Bauer, M., Gormley, N., Gilbert, J. 0000000976 00000 n Region-specific primers, tagged with Illumina adapters, were used to amplify the . `)d FLv,r""3K9& 0000066413 00000 n . hU{4y;TkTfdg;DJE! Modifications to primer degeneracy were done by the labs of Jed Furhman (Parada et al., 2016) and Amy Apprill (Apprill et al., 2015). For specific trademark information, see www.illumina.com/company/legal.html. Note: The 5 adapter sequence/index sequencing primer has an extra GCT at its 3 end compared to Illuminas usual index primer sequences. 2022 Illumina, Inc. All rights reserved. 0000017088 00000 n These libraries are compatible with paired-end sequencing on the Illumina sequencing platforms. Apps, DRAGEN 0000091276 00000 n Illumina offers an extensive assortment of easy-to-use next-generation sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. Products, DRAGEN v4.0 release enables machine learning by default, providing increased accuracy out of the box, Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencing, Whole-exome sequencing kit with library prep, hybridization reagents, exome probe panel, size selection beads, and indexes, See what is possible through the latest advances in high-throughput sequencing technology, View the unveiling of our newest technologies and products on-demand, recorded live at the Illumina Genomics Forum, Get instructions for using Illumina DRAGEN Bio-IT Platform v4.0, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. 0000114588 00000 n BaseSpace W3500) or MoBio (cat. Customer Dashboard, Infrastructure A. Host: https://www.illumina.com | Supports customers processing at a different plexity from what is recommended. 0000052744 00000 n Furthermore, NGS offers the ability to combine multiple samples in a sequencing run. 0000066161 00000 n 0000065952 00000 n trailer The result: a workflow that is even easier to use, scalable for any size lab, requires a small number of steps, and has a fast workflow time. 0000043929 00000 n 0000087498 00000 n Title: 16S Metagenomic Sequencing Library Preparation (Modified version of Illumina) Created Date: 20221014172109Z 0000006237 00000 n we will discuss targeted metagenomics and shotgun metagenomics sequencing, including the following topics: - an overview of metagenomics - introduction to experimental approaches -. Products 16S Metagenomic Sequencing Library Preparation The 16S Illumina Demonstrated Library Prep Guide and links to an example 16S dataset from libraries generated with the protocol and run on the MiSeq with v3 reagents. The demonstrated Illumina protocol for 16S metagenomic sequencing library preparation targets the V3 and V4 variable regions of the 16S rRNA gene. 0000013121 00000 n 0000120850 00000 n 0000010697 00000 n Sequence Hub, BaseSpace For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. In addition, youll experience superior support across the entire workflow because our customer support team will have more information to help you troubleshoot your sequencing runs. 0000096585 00000 n As of 2018, the EMP has switched to a low-cost, miniaturized (5-l volume), high-throughput (384-sample) amplicon library preparation method with the Echo 550 acoustic liquid handler (Minich et al., 2018). It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. With a PCR-based workflow and ease of use for users new to NGS, amplicon library prep can measure thousands of targets simultaneously. The hypervariable 16S rRNA region between the primer binding sequences is replaced with artificial sequences originating from Arabidopsis thaliana. Supplementary Table 1 Summary of Illumina sequencing data generated in this study * All rows represent separately collected biological samples, i.e. 0000074359 00000 n If working with more than 96 samples, the pool may need to be split evenly for cleaning and then recombined. The total number of reads from the 11 samples was 413 million (Supplementary Table S8). 0000115311 00000 n Illumina 16S Metagenomics Sequencing Protocol Author: Illumina Subject: Get answers to frequently asked questions about library preparation for 16S metagenomics studies. 0000071271 00000 n The 16S Metagenomic Sequencing Library Preparation Guide leads users through each 0000087420 00000 n 0000049409 00000 n 0000022852 00000 n 0000115233 00000 n 0000004881 00000 n The NEXTFLEX 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. Software Suite, BaseSpace Download Guide Metagenomic Sequencing Research Articles The Mysterious World of Microbes HWKo@6'$sA r 0000000016 00000 n Highthroughput sequencing of the 16S rRNA gene on the Illumina platform is commonly used to assess microbial diversity in environmental samples. Order Now. The NEXTflex 16S V4 kit is designed for sequencing of the 4th hypervariable domain of microbial 16S rRNA, a very specific phylogenetic marker. 0000113260 00000 n 0000074088 00000 n 62 0 obj <>stream Learn how to convert library concentration from ng/l to nM for some Illumina library preparation methods. 0000088040 00000 n 0000052002 00000 n As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Objectives We will discuss the comprehensive workflow for a metagenomics sequencing project - What is meta no. It is optimized to target the V3 and V4 regions of the 16S rRNA gene, although it can be adapted to target other variable regions. Learn when library normalization is required, and how to perform the necessary steps. Benefit from the best customer support using the entire Illumina workflow. The most important similarities and differences of 16S microbiome sequencing in 20 fecal rat samples were described. 0000096663 00000 n A., Smith, G., & Knight, R. (2012). Tax Reg: 105-87-87282 | 0000009516 00000 n 0000114666 00000 n 0000065754 00000 n Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price, All Software & Informatics 0000096624 00000 n The NextSeq 1000 and NextSeq 2000 Systems support emerging and mid-throughput sequencing applications as well as a broad range of methods such as exome sequencing, target enrichment, single-cell profiling, transcriptome sequencing, and more. 0000007162 00000 n (2016). Perform taxonomic . 0000093807 00000 n 0000118070 00000 n 0000041406 00000 n Even those new to NGS will find the workflows and applications are easy to adopt. Low Input Requirements for 16S rRNA Sequencing Bio-IT Platform, TruSight Bioz Stars score: 80/100, based on 1 PubMed citations. Find out how to quantify and validate final libraries for a successful sequencing run. 16S/18S/ITS Amplicon Metagenomic Sequencing is an ultra-deep DNA sequencing method that focuses on . Not for import or sale to the Australian general public. %%EOF 29 34 Send an aliquot for sequencing along with sequencing primers listed below. PerkinElmer offers NEXTFLEX 16S Amplicon-Seq Kits for this purpose, which are optimized library prep kits developed to simplify 16S rRNA sequencing on the Illumina sequencing platforms. Considering the importance of identifying foodborne pathogens during outbreaks, this study demonstrates the use of high-throughput sequencing to understand the enrichment and identification pathogens in . 0000052305 00000 n 0000088311 00000 n 0000003282 00000 n z^4ME%"}vwxvtH='\$UoyVm+aWs P6(&*Qo"{EUd{4Ev(C";o VY/(m #/R -s^^zSH%l0n;\YOx)=/+C_y! h See the page Primer Ordering and Resuspension for more information. Apps, DRAGEN 0000068757 00000 n QIAseq 16S/ITS Smart Control is a synthetic DNA that can be used as a positive control for library construction steps as it contains the primer binding sites from Escherichia coli. 0000088503 00000 n no. Sequence Hub, BaseSpace Created Date: 9/5/2017 2:34:08 PM 0000018153 00000 n ITS analysis with NGS enables rapid fungal identification to help advance our understanding of the mycobiome. Do not combine amplicons from different samples at this point. 0000011932 00000 n Here we used a modified custom primer sequencing approach to test the fidelity of the . | 0000030603 00000 n NovaSeq 6000 Reagent Kits v1.5. from sample prep through library construction & sequencing. 0000114853 00000 n 0000009321 00000 n 0000013281 00000 n Library Prep & Array Kit Selector; Gene Panel & Array Finder . Analyzes DNA from amplicon sequencing of prokaryotic 16S small subunit rRNA genes. 0000163468 00000 n 0000113807 00000 n Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. 0000068679 00000 n (2012). B; Illumina, USA). The ligation process prepares NGS libraries by fragmenting a genomic DNA or cDNA sample and ligating specialized adapters to both fragment ends. 1) Perform 16S metagenomics on complex sample types (stool, milk, etc.) -ExQMsA. A 16S amplicon library was prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation Guide 1 with minor modifications. Higher amounts can be used if the final pool will be gel-isolated or when working with low-biomass samples. 0000007625 00000 n Variant Interpreter, MyIllumina (2012). They can reduce false-positive variant calls while increasing variant detection sensitivity. Illumina MiSeq Sequencing of Bacterial 16S rRNA Amplicons. Metagenomics is the study of the functional genomes of microbial communities while 16S sequencing offers a phylogenetic survey on the diversity of a single ribosomal gene, 16S rRNA. P11496; follow manufacturers instructions). 31`&"BWL+hb^B&NoMoejiGRirI *r3?8fXU//$ 0000014618 00000 n Improved Q30 score, support for UMIs, extended shelf life, and support for Illumina DNA PCR-Free Library Prep. Illumina library prep protocols accommodate a range of throughput needs, from lower-throughput protocols for small labs to fully automated library preparation workstations for large laboratories or genome centers. Order Now ITS analysis with NGS enables rapid fungal identification to help advance our understanding of the mycobiome. 0000012033 00000 n 0000004805 00000 n Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in . 0000007007 00000 n Environmental Microbiology, 18(5), 14031414. 0000079663 00000 n Quantify amplicons with Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher/Invitrogen cat. High-throughput miniaturized 16S rRNA amplicon library preparation reduces costs while preserving microbiome integrity. 0000021903 00000 n endstream endobj 39 0 obj <>stream Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. You will need to make your sample more complex by adding 510% PhiX to your run. The MiniSeq, Illumina's latest benchtop sequencer, enables more costefficient DNA sequencing relative to larger Illumina sequencing platforms (e.g., MiSeq). Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. Triple-indexing greatly reduces the number of long custom DNA oligos required for library preparation, while the inclusion of variable length heterogeneity spacers mi 0000044278 00000 n This enables the usage of various reverse primer constructs to obtain longer amplicons, for example the V4V5 region using reverse primer 926R(Quince et al., 2011; Parada et al., 2016). vnvS;m6npNs@=cQB81O+>M=KtU +t$l8xYBe 8%c@,b n\0LE $:Opr0ES4ZoLoeAb57-f$!% urv w&SL&&O=3}8IKG.OWkFRuI{Z 0000025232 00000 n 2) Develop core lab SOPs for single-cell isolation using . The sequencing library was constructed according to the 16S metagenomic sequencing library preparation methods (Illumina) . Amplify samples in triplicate, meaning each sample will be amplified in 3 replicate 25-L PCR reactions. Up to 384 unique dual-index libraries can be prepared for a single NGS run The 16S ribosomal RNA gene (16S rRNA) is a common target for microbial community classification in metagenomic studies. 0000002191 00000 n 16S rRNA NGS allows microbiologists to achieve genus-level sensitivity for metagenomic surveys of bacterial populations. The foundation to discovery and insights. They offer an intuitive workflow with . 0000002324 00000 n 0000114343 00000 n AATGATACGGCGACCACCGAGATCTACACGCT 0000057314 00000 n The quality of the extracted gDNA was sufficiently high to produce a sequencing library for Illumina sequencing using commercially available kits. Bio-IT Platform, TruSight 0000113437 00000 n DNA was extracted from the frozen sediment-river water samples (5 mL) using a Powersoil DNA isolation kit (MO BIO Laboratories). We feel it's necessary to explicitly state this as . !'^ A comprehensive suite of library preparation and target enrichment kits for DNA, RNA, and epigenetic sequencing studies, optimized for Illumina sequencers. Barcodes are now on the forward primer 515F (Parada et al., 2016). Always check the primer sequence in addition to the primer name. 0000001924 00000 n Genomic . 0000015839 00000 n . 0000117989 00000 n BMC Bioinformatics 12: 38. 0000005806 00000 n %PDF-1.4 % wxB.&Z@6 8]tL_pP@dkY"pc0t_Id@|}>~\{1kjBd9n()Ev;p,M Even those new to NGS will find the workflows and applications are easy to adopt. 0000002456 00000 n Improved bacterial 16S rRNA gene (V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015). 0000041322 00000 n 0000038806 00000 n Therefore we now use the original names following by the lead author to avoid ambiguity, i.e., 515F (Parada), 806R (Apprill), and 926R (Quince). Breakthrough technology in our library prep helps you get to answers in less time. 0000002600 00000 n 0000008395 00000 n Our partners have developed both high- and low-throughput walk-away automation methods that span our library prep portfolio. Sequencing with ViroCap enrichment took >138 h and included some resource-intensive and labor-intensive steps (e.g., sequencing library construction, computational analysis of the sequence data). hb```f``)c`e`H``@ +I3Guy]270 0000046801 00000 n 0000062512 00000 n Improved Q30 score, support for UMIs, extended shelf life, and support for Illumina DNA PCR-Free Library Prep. 0000118229 00000 n A., Jansson, J. K., Caporaso, J. G., Fuhrman, J. The 16S protocol detailed here is designed to amplify prokaryotes (bacteria and archaea) using paired-end 16S community sequencing on the Illumina platform. Library Prep Kit Selector High-Throughput Automation Solutions . Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. 0000038728 00000 n Run amplicons from each sample on an agarose gel. 0000013656 00000 n 0000005907 00000 n Interpretation, Certificates (CofC, CofA) and Master Lot Sheets, AmpliSeq for Illumina Cancer Hotspot Panel v2, AmpliSeq for Illumina Comprehensive Cancer Panel, Breast Cancer Target Identification with High-Throughput NGS, The Complex World of Pan-Cancer Biomarkers, Microbiome Studies Help Refine Drug Discovery, Identifying Multidrug-Resistant Tuberculosis Strains, Investigating the Mysterious World of Microbes, IDbyDNA Partnership on NGS Infectious Disease Solutions, Infinium iSelect Custom Genotyping BeadChips, 2020 Agricultural Greater Good Grant Winner, 2019 Agricultural Greater Good Grant Winner, Gene Target Identification & Pathway Analysis, TruSeq Methyl Capture EPIC Library Prep Kit, Genetic Contributions of Cognitive Control, Challenges and Potential of NGS in Oncology Testing, Partnerships Catalyze Patient Access to Genomic Testing, Patients with Challenging Cancers to Benefit from Sequencing, NIPT vs Traditional Aneuploidy Screening Methods, SNP Array Identifies Inherited Genetic Disorder Contributing to IVF Failures, NIPT Delivers Sigh of Relief to Expectant Mother, Education is Key to Noninvasive Prenatal Testing, Study Takes a Look at Fetal Chromosomal Abnormalities, Rare Disease Variants in Infants with Undiagnosed Disease, A Genetic Data Matchmaking Service for Researchers, Using NGS to Study Rare Undiagnosed Genetic Disease, Progress for Patients with Rare and Undiagnosed Genetic Diseases, 1 to 1000 ng standard quality RNA; 10 ng for optimal performance and FFPE samples, 10 ng standard quality RNA; 20 ng RNA for low quality / degraded / FFPE. 0000019572 00000 n 0000113983 00000 n 0000233823 00000 n 0000006083 00000 n 0000120928 00000 n Retailer Reg: 2019--2018 | 0000009110 00000 n 0000003320 00000 n Quality, precision, and ease of use in every step with our library prep portfolio advancements. mSystems, 1(1), e0000915. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. startxref 0000084827 00000 n 0000036119 00000 n Get more out of your samples, reduce hands-on time, and save money. 12500; follow manufacturers instructions). Primers 515F-806R target the V4 region of the 16S SSU rRNA. Clean amplicon pool using MoBio UltraClean PCR Clean-Up Kit (cat. Analysis, Biological Data Following Illumina 16S rRNA amplicon sequencing, the sequences found were bioinformatically processed, analyzed, and visualized in KRONA charts (for details, see the Section 2 ). More Info | Library Prep & Array Kit Selector . 0000088233 00000 n Learn More Kits Reagents Library Preparation KitsSequencing ReagentsMicroarray KitsClinical Research ProductsIVD ProductsAll Kits Reagents TruSight. They commonly involve sequencing the 16S ribosomal RNA (rRNA) gene for taxonomic classification. Not for import or sale to the Australian general public. Short (99% Q20 accuracy. 0000018588 00000 n 0000113136 00000 n no. Background: Shotgun metagenomic sequencing reveals the potential in microbial communities. <]/Prev 838145/XRefStm 4881>> 0000077047 00000 n The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. 0000006391 00000 n The protocol from Illumina's 16S Metagenomic Library Prep Guide (Illumina 2013) was adapted for this experiment. %PDF-1.4 % Updated sequences: 515F (Parada)806R (Apprill), forward-barcoded: Original sequences: 515F (Caporaso)806R (Caporaso), reverse-barcoded: Reverse complement of 3 Illumina adapter, PCR-grade water from Sigma (cat. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. 0000115046 00000 n Files Name Type & Size Date 16S Metagenomic Sequencing Library Preparation (15044223 B) PDF (< 1 MB) Nov 27, 2013 (2012). 0000007192 00000 n NGS library prep - 16s Metagenomic Libraries for 16S Metagenomic Sequencing are prepared using Illumina's demonstrated protocol. This technology uses bead-bound transposomes for a more uniform reaction compared to in-solution tagmentation reactions. Library preparation with continual innovation. Sequencing by synthesis (SBS) technology is used by the Illumina NovaSeq 6000 platform to sequence the library and, once sequencing is . 0000001790 00000 n (2016) based upon the original constructs generated by Caporaso et al. c.V}Jlmc Here, we provide the most comprehensive study of performance and bias inherent to a 16S rRNA gene amplicon sequencing method to date. For specific trademark information, see www.illumina.com/company/legal.html. Diversity was high with an InvSimpson index of 63 (Range 5 - 496, SD 121).
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